5W0V
Crystal structure of full-length Kluyveromyces lactis Kap123 with histone H4 1-34
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2016-12-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.793, 87.697, 101.623 |
| Unit cell angles | 79.59, 81.45, 71.70 |
Refinement procedure
| Resolution | 33.787 - 2.821 |
| R-factor | 0.1724 |
| Rwork | 0.170 |
| R-free | 0.23390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vch |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.033 |
| Data reduction software | HKL-2000 (HKL2000_v714-Linux) |
| Data scaling software | HKL-2000 (HKL2000_v714-Linux) |
| Phasing software | PHENIX (1.11.1_2575) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.930 |
| High resolution limit [Å] | 2.821 | 2.821 |
| Rmerge | 0.044 | 0.632 |
| Rpim | 0.045 | 0.479 |
| Number of reflections | 59828 | 5938 |
| <I/σ(I)> | 20.7 | 1.54 |
| Completeness [%] | 98.9 | 98 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296.15 | 0.1M sodium cacodylate (pH 6.5), 0.2M sodium acetate, 30% PEG 4000 and 5% Jeffamin M-600 |
