5W0O

Structure of human TUT7 catalytic module (CM) in complex with dsRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	66.825, 80.851, 181.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.458 - 2.488
R-factor	0.2079
Rwork	0.205
R-free	0.25420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w0m
RMSD bond length	0.006
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	181.832	2.510
High resolution limit [Å]	2.488	2.488
Rmerge	0.049	0.734
Number of reflections	35240
<I/σ(I)>	19.4
Completeness [%]	99.5
Redundancy	4.7
CC(1/2)	0.999	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.2 M ammonium acetate, 0.1 M trisodium citrate, pH 5.5, 14% PEG400