5W0K

Crystal structure of EBV gHgL/CL40/gp42 N-domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9998
Spacegroup name	P 21 21 21
Unit cell lengths	96.380, 133.130, 254.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.763 - 3.100
R-factor	0.2445
Rwork	0.242
R-free	0.29910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3phf 2xqy
RMSD bond length	0.003
RMSD bond angle	0.627
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.190
High resolution limit [Å]	3.100
Number of reflections	60129
<I/σ(I)>	11.7
Completeness [%]	100.0
Redundancy	14.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.25 M sodium sulfate, 0.1 M Bis-tris propane pH 8.5, 20 % (w/v) PEG 4000