5W06

HUMAN TISSUE FACTOR IN COMPLEX WITH ANTIBODY M1587

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-17
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	68.100, 175.560, 63.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.18822
Rwork	0.186
R-free	0.23495
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uj3 4m7k
RMSD bond length	0.007
RMSD bond angle	1.142
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.670
High resolution limit [Å]	2.600	2.600
Rmerge	0.088	0.403
Number of reflections	23936	1678
<I/σ(I)>	16	4.7
Completeness [%]	99.5	94.7
Redundancy	6.9	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1 M SODIUM ACETATE PH 4.5, 18% PEG 3K, 0.2 M AMMONIUM ACETATE