5VZF

Post-catalytic complex of human Polymerase Mu (W434A) mutant with incoming dTTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	59.866, 68.690, 110.224
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.790 - 1.650
R-factor	0.1707
Rwork	0.169
R-free	0.19520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.011
RMSD bond angle	1.136
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.680
High resolution limit [Å]	1.650	1.650
Rpim	0.027	0.299
Number of reflections	55355	2725
<I/σ(I)>	26.33	2.71
Completeness [%]	99.7	99.1
Redundancy	7.1	6.1
CC(1/2)		0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	90mM HEPES pH 7.5, 18% PEG4K