5VZC

Post-catalytic complex of human Polymerase Mu (G433S) mutant with incoming dTTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	60.112, 68.716, 110.993
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.821 - 1.552
R-factor	0.1666
Rwork	0.165
R-free	0.19020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.009
RMSD bond angle	1.043
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Rpim	0.034	0.379
Number of reflections	66859	3250
<I/σ(I)>	23.64	2.29
Completeness [%]	99.7	97.7
Redundancy	6.6	3.5
CC(1/2)		0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	90mM HEPES pH 7.5, 18% PEG4K