5VYS
Crystal structure of the WbkC N-formyltransferase (C47S variant) from Brucella melitensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-25 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 65.927, 65.927, 234.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.965 - 2.200 |
| R-factor | 0.18874 |
| Rwork | 0.185 |
| R-free | 0.25784 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vyr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.592 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.096 | 0.405 |
| Number of reflections | 27237 | 3263 |
| <I/σ(I)> | 12.3 | 4 |
| Completeness [%] | 99.6 | 98.4 |
| Redundancy | 14.1 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 19-25%PEG-5000, 5 mM GDP-perosamine, 5 mM folinic acid, 100 mM MOPS |
