5VYE

Crystal Structure of L-Threonine Aldolase from Pseudomonas putida

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	198.167, 186.960, 53.269
Unit cell angles	90.00, 98.92, 90.00

Refinement procedure

Resolution	26.754 - 2.275
R-factor	0.1808
Rwork	0.180
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lnj
RMSD bond length	0.015
RMSD bond angle	1.268
Data reduction software	XDS
Phasing software	PHENIX ((1.11.1_2575: ???))
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.755	2.356
High resolution limit [Å]	2.275	2.275
Rmerge	0.088
Rpim	0.037
Number of reflections	172206	87029
<I/σ(I)>	14.5
Completeness [%]	99.4	99
Redundancy	6.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2.1 M sodium formate, 0.1 M sodium acetate trihydrate pH 4.6