5VXQ

X-Ray crystallography structure of the parallel stranded duplex formed by 5-rA5-dA-rA5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	77
Detector technology	CCD
Collection date	2015-05-01
Detector	RAYONIX MX-300
Wavelength(s)	1.28206
Spacegroup name	P 41 21 2
Unit cell lengths	22.799, 22.799, 164.071
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.580 - 1.002
Rwork	0.129
R-free	0.15980
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	PHENIX (1.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.580
High resolution limit [Å]	1.002	1.002
Number of reflections	23843
<I/σ(I)>	17.65
Completeness [%]	96.4
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP, VAPOR DIFFUSION	5	295	0.5 uL 5-rA5-dA-rA5 and RRM23 (98-269) in 10 mM HEPES, 100 mM sodium chloride, 2 mM DTT, pH 7.0 + 0.5 uL 1.6 M ammonium sulfate, 0.1 M citric acid, pH 5.0