5VX8

VP8* of P[6] Human Rotavirus RV3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 1
Unit cell lengths	27.790, 43.100, 65.740
Unit cell angles	94.86, 99.40, 90.22

Refinement procedure

Resolution	27.410 - 2.000
R-factor	0.2107
Rwork	0.208
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aen
RMSD bond length	0.004
RMSD bond angle	0.694
Data reduction software	iMOSFLM (7.0.9)
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX (1.11.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.410
High resolution limit [Å]	2.000	2.000
Rmerge	0.071	0.102
Number of reflections	33614
<I/σ(I)>	7.8
Completeness [%]	90.5
Redundancy	2.1
CC(1/2)	0.986	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.7 M ammonium sulfate, 0.1 M Tris, pH 7.0