5VX1
Bak L100A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.497, 39.488, 108.107 |
| Unit cell angles | 90.00, 91.25, 90.00 |
Refinement procedure
| Resolution | 36.027 - 1.224 |
| R-factor | 0.1684 |
| Rwork | 0.168 |
| R-free | 0.19290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.330 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.027 |
| High resolution limit [Å] | 1.224 |
| Rmerge | 0.046 |
| Number of reflections | 98200 |
| <I/σ(I)> | 14.94 |
| Completeness [%] | 95.0 |
| Redundancy | 3.8 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 281 | 1M sodium malonate-malonic acid pH 7.0 and 10 % DL-malate-MES-tris pH 9 |
