5VW5
Nicotinamide soak of Y316A mutant of corn root ferredoxin:NADP+ reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.400, 59.400, 187.451 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.720 - 1.953 |
| R-factor | 0.1353 |
| Rwork | 0.130 |
| R-free | 0.18010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lo8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.088 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.720 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rpim | 0.013 | 0.038 |
| Number of reflections | 24769 | 1325 |
| <I/σ(I)> | 34.3 | 9.6 |
| Completeness [%] | 85.8 | 32.5 |
| Redundancy | 9.1 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 22-24% PEG 8000, 0.1 M sodium cacodylate (pH 6-7), 0.18-0.22 M magnesium acetate, 100 mM nicotinamide |
