5VW4
Nicotinamide soak of Y316S mutant of corn root ferredoxin:NADP+ reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 58.894, 58.894, 184.812 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.004 - 1.351 |
R-factor | 0.1385 |
Rwork | 0.135 |
R-free | 0.17320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lo8 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.013 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.000 | 1.420 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.091 | 0.241 |
Number of reflections | 75008 | 9295 |
<I/σ(I)> | 6.2 | 1.7 |
Completeness [%] | 91.0 | 78.4 |
Redundancy | 5.8 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 22-24% PEG 8000, 0.1 M sodium cacodylate (pH 6-7), 0.18-0.22 M magnesium acetate, 100 mM nicotinamide |