5VVW

Structure of MurC from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-18
Detector	RAYONIX MX300HE
Wavelength(s)	0.97949
Spacegroup name	C 1 2 1
Unit cell lengths	284.820, 109.400, 108.580
Unit cell angles	90.00, 112.38, 90.00

Refinement procedure

Resolution	29.812 - 2.300
R-factor	0.189
Rwork	0.189
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hv4
RMSD bond length	0.008
RMSD bond angle	0.916
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2744: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.812
High resolution limit [Å]	2.300
Rmerge	0.080
Number of reflections	136319
<I/σ(I)>	12.46
Completeness [%]	99.7
Redundancy	4.6
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Morpheus A2: 60mM Divalents, (0.3M Magnesium chloride hexahydrate; 0.3M Calcium chloride dihydrate), 100mM Imidazole; MES monohydrate (acid), 20% v/v Ethylene glycol; 10 % w/v PEG 8000.