5VU9

TNA polymerase, translocated product

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	69.139, 110.920, 151.069
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.852 - 2.050
R-factor	0.1914
Rwork	0.190
R-free	0.23520
RMSD bond length	0.009
RMSD bond angle	0.927
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_2481: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.850	2.110
High resolution limit [Å]	2.050	2.050
Rmerge	0.034	0.789
Number of reflections	69934
<I/σ(I)>	11
Completeness [%]	94.9
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M sodium sulfate, 0.1 M MES, pH 5.8, 12% PEG3350, Silver Bullets Bio (Hampton Research) additive #56