5VTW
Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin G225M/L226T/S228A mutant in complex with 6'-SLN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0331 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 208.650, 131.530, 72.728 |
Unit cell angles | 90.00, 98.05, 90.00 |
Refinement procedure
Resolution | 110.950 - 2.350 |
R-factor | 0.19411 |
Rwork | 0.193 |
R-free | 0.21270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fnk |
RMSD bond length | 0.009 |
RMSD bond angle | 1.440 |
Data scaling software | HKL-2000 (712) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rpim | 0.060 | 0.380 |
Number of reflections | 79362 | 7097 |
<I/σ(I)> | 13.5 | 1.3 |
Completeness [%] | 98.9 | 97.4 |
Redundancy | 5.3 | 4.7 |
CC(1/2) | 1.000 | 0.630 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M sodium cacodylate pH 6.5, 5% PEG 8000, and 38% 2-methyl-2,4-pentanediol |