5VTE
Hetero antiparallel coiled coil hexamer formed by de novo peptides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 48.970, 48.970, 141.821 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.274 - 2.023 |
R-factor | 0.2103 |
Rwork | 0.207 |
R-free | 0.23700 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.590 |
Data reduction software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.274 | 2.096 |
High resolution limit [Å] | 2.023 | 2.023 |
Rmerge | 0.045 | 0.091 |
Number of reflections | 8400 | 342 |
<I/σ(I)> | 10.63 | 3.76 |
Completeness [%] | 93.0 | 89 |
Redundancy | 5.4 | 4.3 |
CC(1/2) | 0.993 | 0.977 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 298 | 0.1 M sodium formate, pH 6.75, PEG 3350 24% |