5VTB

Crystal structure of RBBP4 bound to BCL11a peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	51.301, 85.264, 87.968
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.320 - 2.400
R-factor	0.203
Rwork	0.201
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r7a
RMSD bond length	0.010
RMSD bond angle	1.080
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.390	6.510	2.400
Rmerge	0.115	0.047	0.566
Rmeas	0.123	0.051	0.604
Rpim	0.043	0.018	0.209
Number of reflections	15853
<I/σ(I)>	6.4
Completeness [%]	99.7	99.3	98.7
Redundancy	8.2	7.6	8
CC(1/2)		0.997	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% PEG2000 MME, 0.1 M potassium thiocyanate