5VT9
Myosin Light chain 1 and MyoA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.127 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.990, 64.390, 65.150 |
| Unit cell angles | 90.00, 97.72, 90.00 |
Refinement procedure
| Resolution | 40.618 - 1.850 |
| R-factor | 0.1928 |
| Rwork | 0.191 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qac |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.949 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.560 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.076 | 0.627 |
| Number of reflections | 28665 | 1749 |
| <I/σ(I)> | 15.3 | 2.9 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.9 uL 8 mg/mL protein + 1.35 uL reservoir (0.1 M Bis-Tris, pH 5.5, 25% PEG3350) |
