5VT8
Crystal Structure of Mouse Cadherin-23 EC24-25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.613, 99.387, 157.425 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 47.390 - 2.920 |
| R-factor | 0.20915 |
| Rwork | 0.208 |
| R-free | 0.22142 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uz8 2ee0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.438 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Rmerge | 0.107 | 0.422 |
| Rmeas | 0.145 | 0.568 |
| Rpim | 0.097 | 0.377 |
| Number of reflections | 18772 | 854 |
| <I/σ(I)> | 6.37 | 1.76 |
| Completeness [%] | 94.3 | 93.8 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.998 | 0.769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 277 | 0.1 M TRIS pH 7.9 10% PEG 8000 |
