5VSX

Structure of the Ubl domain of Sacsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-29
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	51.901, 62.606, 90.834
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.951 - 2.100
R-factor	0.1895
Rwork	0.187
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vsz SeMet-L78M mutant
RMSD bond length	0.011
RMSD bond angle	1.270
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.060	0.042	0.289
Total number of observations	92223
Number of reflections	8861
<I/σ(I)>	20.7
Completeness [%]	98.8	96.2	82.1
Redundancy	10.4	9.7	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.7	293	0.1 M Bicine pH 8.7, 0.2 M NaCl, 20% sucrose