5VRY

Human DNA polymerase beta 8-oxoG:dC extension with dTTP after 20 s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-12
Detector	RAYONIX MX300HE
Wavelength(s)	0.97931
Spacegroup name	P 1 21 1
Unit cell lengths	50.900, 80.100, 55.600
Unit cell angles	90.00, 107.20, 90.00

Refinement procedure

Resolution	48.620 - 1.900
R-factor	0.2048
Rwork	0.203
R-free	0.24350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rpx
RMSD bond length	0.005
RMSD bond angle	1.148
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.620	48.620	2.200
High resolution limit [Å]	1.900	12.000	1.900
Rmerge	0.050	0.021	0.234
Rmeas	0.069	0.029	0.317
Number of reflections	63931	131	23277
<I/σ(I)>	11.11	29.41	3.36
Completeness [%]	96.5	49.2	98.7
Redundancy	1.935	1.634	1.924
CC(1/2)	0.998	0.997	0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM imidazole pH 7.0-7.5, 350 mM sodium acetate, 16-18% PEG 3350