5VRI

Crystal structure of SsoPox AsA6 mutant (F46L-C258A-W263M-I280T) - closed form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	87.170, 103.610, 152.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.713 - 2.150
R-factor	0.17433
Rwork	0.172
R-free	0.21351
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vc5
RMSD bond length	0.003
RMSD bond angle	0.731
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.713
High resolution limit [Å]	1.400
Number of reflections	121915
<I/σ(I)>	9.07
Completeness [%]	96.1
Redundancy	3.44

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	20-30 % (w/v) PEG 8000 and 50 mM Tris-HCl buffer (pH 8).