5VR9

CH1/Ckappa Fab based on Matuzumab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	77
Detector technology	CCD
Collection date	2015-12-09
Detector	RAYONIX MX225HE
Wavelength(s)	0.97931
Spacegroup name	I 41 2 2
Unit cell lengths	152.046, 152.046, 220.619
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.2116
Rwork	0.210
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c08
RMSD bond length	0.008
RMSD bond angle	1.221
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.260
High resolution limit [Å]	2.150	2.150
Rmerge	0.076	0.551
Number of reflections	70553	10174
<I/σ(I)>	22.6
Completeness [%]	100.0	100
Redundancy	13.7	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	see publication for details