5VR9
CH1/Ckappa Fab based on Matuzumab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-12-09 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 152.046, 152.046, 220.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.150 |
| R-factor | 0.2116 |
| Rwork | 0.210 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c08 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.221 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.260 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.076 | 0.551 |
| Number of reflections | 70553 | 10174 |
| <I/σ(I)> | 22.6 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.7 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | see publication for details |
