5VP3

Light-sensitive photoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-28
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 65
Unit cell lengths	72.614, 72.614, 81.429
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.177 - 2.151
R-factor	0.1626
Rwork	0.161
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bpj
RMSD bond length	0.012
RMSD bond angle	1.185
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.710	2.220
High resolution limit [Å]	2.150	2.150
Rmerge	0.116	0.637
Rpim	0.029	0.169
Number of reflections	13301	1090
<I/σ(I)>	17.8	4.1
Completeness [%]	99.5	94.5
Redundancy	16.9	14.3
CC(1/2)	0.999	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Potassium Tiocyanate, 20% (w/v) PEG 3350