5VP2

Crystal structure of the Thermus thermophilus 70S ribosome in complex with madumycin II and bound to mRNA and A-, P- and E-site tRNAs at 2.8A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	209.940, 451.230, 622.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	155.610 - 2.800
R-factor	0.2315
Rwork	0.229
R-free	0.27840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.652
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	155.610	155.610	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.146	0.049	1.067
Rmeas	0.168	0.055	1.272
Total number of observations	5230080
Number of reflections	1382659	14827	102242
<I/σ(I)>	7.83	24.91	1.09
Completeness [%]	96.6	87.4	97
Redundancy	3.783	4.487	3.106
CC(1/2)	0.987	0.996	0.239

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME