5VNY

Crystal structure of DM14-3 domain of Lgd

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	29.670, 29.670, 139.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.778 - 1.101
R-factor	0.1703
Rwork	0.170
R-free	0.17820
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.897
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.130
High resolution limit [Å]	1.100	1.100
Number of reflections	46405
<I/σ(I)>	11.61
Completeness [%]	96.4
Redundancy	5.8
CC(1/2)	0.998	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	30% PEG2000 MME, Tris, pH 8.0