5VNG

Crystal structure of Sec23a/Sec24a/Sec22 complexed with a C-terminal II sorting motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	PIXEL
Collection date	2015-07-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	148.262, 97.102, 130.032
Unit cell angles	90.00, 90.19, 90.00

Refinement procedure

Resolution	48.962 - 2.600
R-factor	0.2011
Rwork	0.199
R-free	0.25710
RMSD bond length	0.002
RMSD bond angle	0.537
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	54087
<I/σ(I)>	1.1
Completeness [%]	98.2
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50mM HEPES PH 7.8, 0.5M NaAc and 12% PEG4K