5VN7

Structure of bacteriorhodopsin from crystals grown at 20 deg Celcius using GlyNCOC15+4 as an LCP host lipid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	H 3
Unit cell lengths	107.050, 107.050, 149.860
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.519 - 2.700
R-factor	0.1976
Rwork	0.196
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xji
RMSD bond length	0.004
RMSD bond angle	0.646
Data reduction software	XDS
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.138	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.151	0.645
Number of reflections	17181	1716
<I/σ(I)>	7.4	1.8
Completeness [%]	97.0	96
Redundancy	3	2.4
CC(1/2)	0.988	0.476

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.6	293	1.9-3.0 M sodium/potassium phosphate buffer (pH5.6), 3.5% v/v methylpentanediol and 0.5% w/v OG