5VMX

Kaiso (ZBTB33) zinc finger DNA binding domain in complex with a hemi CpG-methylated DNA resembling the specific Kaiso binding sequence (KBS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 2
Unit cell lengths	44.338, 183.984, 105.072
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.104 - 2.050
R-factor	0.1649
Rwork	0.162
R-free	0.18760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6n
RMSD bond length	0.010
RMSD bond angle	1.217
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.104	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.089	0.473
Number of reflections	27026	2370
<I/σ(I)>	12.28	3.87
Completeness [%]	98.0	87.75
Redundancy	10.3	6.1
CC(1/2)	0.998	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	295	0.1 M Disodium tartrate, 20 % PEG 3350