5VMU

Kaiso (ZBTB33) zinc finger DNA binding domain in complex with a double CpG-methylated DNA resembling the specific Kaiso binding sequence (KBS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 2
Unit cell lengths	43.985, 185.014, 105.108
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.336 - 2.346
R-factor	0.1825
Rwork	0.179
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6n
RMSD bond length	0.010
RMSD bond angle	1.174
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.336	2.430
High resolution limit [Å]	2.346	2.346
Rmerge	0.118	0.629
Number of reflections	17827	1245
<I/σ(I)>	11.64	2.04
Completeness [%]	96.2	67.78
Redundancy	10.5	6.2
CC(1/2)	0.993	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	295	0.1 M Di-sodium tartrate, 20% PEG 3350