5VMO

Crystal structure of grouper iridovirus GIV66:Bim complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 65
Unit cell lengths	71.800, 71.800, 56.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.900 - 1.700
R-factor	0.1622
Rwork	0.160
R-free	0.19970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vmn
RMSD bond length	0.018
RMSD bond angle	1.623
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.840	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.100	1.575
Number of reflections	18346	901
<I/σ(I)>	22.4
Completeness [%]	100.0	99.2
Redundancy	19.9	9.3
CC(1/2)	0.999	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	0.1 M CHES pH 9.5, 20 % PEG 8K