5VLQ
Structure of the TTLL3 Glycylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-24 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9777 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.625, 192.241, 60.743 |
| Unit cell angles | 90.00, 114.33, 90.00 |
Refinement procedure
| Resolution | 48.493 - 2.285 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.23270 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.630 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.890 | 2.320 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Number of reflections | 53787 | |
| <I/σ(I)> | 16.4 | 1.5 |
| Completeness [%] | 97.4 | 75.6 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM Tris-HCl (pH 8), 20% PEG 3350, 200 mM LiSO4. |
