5VLD
Crystal Structure of Medicago truncatula L-Histidinol Dehydrogenase in Complex with L-Histidine and NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-07 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.654, 139.095, 103.558 |
| Unit cell angles | 90.00, 119.47, 90.00 |
Refinement procedure
| Resolution | 48.890 - 2.590 |
| R-factor | 0.22211 |
| Rwork | 0.221 |
| R-free | 0.26437 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kae |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.740 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.890 | 2.750 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.087 | 0.527 |
| Number of reflections | 76791 | 11619 |
| <I/σ(I)> | 10.3 | 2.02 |
| Completeness [%] | 96.4 | 90.9 |
| Redundancy | 2.85 | 2.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 292 | 100 mM MES pH 5.2, 200 mM NaCl, 10% PEG 3350 |
