5VL9

Crystal structure of EilR in complex with eilO DNA element

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2014-10-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97965
Spacegroup name	P 1
Unit cell lengths	54.790, 74.060, 81.360
Unit cell angles	116.80, 105.19, 90.78

Refinement procedure

Resolution	65.288 - 2.160
R-factor	0.1958
Rwork	0.194
R-free	0.22880
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.467
Data reduction software	xia2
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	PHENIX ((dev_2650: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	65.290
High resolution limit [Å]	2.160
Number of reflections	55872
<I/σ(I)>	8.5
Completeness [%]	95.7
Redundancy	3.2
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M Lithium sulphate, 0.1 M Magnesium chloride, 0.1 M Hepes pH 7.5, 20 %PEG 3,350 and 10 % Hexanediol