5VKY

Yeast Tda2 (YER071C) - a dynein light chain family member that works independently of the dynein motor complex and microtubules.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2014-07-13
Detector	RDI CMOS_8M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	50.390, 161.259, 33.258
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.315 - 2.300
R-factor	0.2143
Rwork	0.212
R-free	0.26540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.869
Data reduction software	XDS
Data scaling software	Aimless (0.5.31)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.750	53.750	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.035	0.019	0.195
Rmeas	0.038	0.021	0.212
Rpim	0.015	0.009	0.083
Number of reflections	12771	269	1241
<I/σ(I)>	32.9	60.3	8.4
Completeness [%]	100.0	97.7	100
Redundancy	6.8	5.3	6.4
CC(1/2)	1.000	0.999	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	289	1.6 M ammonium sulfate, 50 mM Bis-Tris, pH 5, 15% xylitol