5VKO

SPT6 tSH2-RPB1 1468-1500 pT1471, pS1493

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-11-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	42.448, 102.102, 115.743
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.453 - 1.800
R-factor	0.1649
Rwork	0.162
R-free	0.19750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3psj
RMSD bond length	0.009
RMSD bond angle	1.013
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.7.15)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.453	40.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.093	0.041	1.119
Rmeas	0.099	0.044	1.238
Rpim	0.035	0.015	0.518
Total number of observations	186789
Number of reflections	23766
<I/σ(I)>	13
Completeness [%]	99.9	99.7	99
Redundancy	7.9	8.5	5.2
CC(1/2)		0.999	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	10% iso-Propanol, 0.1 M Na Hepes pH 7.5, 20% PEG 4000