5VKE

Open conformation of KcsA deep-inactivated

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	150
Detector technology	PIXEL
Collection date	2009-06-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	I 4
Unit cell lengths	156.195, 156.195, 74.172
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.820 - 2.370
R-factor	0.1969
Rwork	0.196
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	35FW
RMSD bond length	0.003
RMSD bond angle	0.636
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.820
High resolution limit [Å]	2.370
Number of reflections	36377
<I/σ(I)>	2.9
Completeness [%]	99.8
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG400, 50 mM magnesium acetate, 50 mM sodium acetate