5VK7
aspartate aminotransferase pH 4.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-01-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.732, 125.249, 131.116 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| Rwork | 0.185 |
| R-free | 0.22500 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.052 | 0.376 |
| Number of reflections | 69048 | 7027 |
| <I/σ(I)> | 24.2 | 3.4 |
| Completeness [%] | 94.4 | 97.9 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5.4 | 277.15 | 40 mM sodium acetate 8% PEG 6,000 |
