5VK6

Open conformation of KcsA non-inactivating E71A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	293
Detector technology	PIXEL
Collection date	2009-06-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	I 4
Unit cell lengths	156.544, 156.544, 74.618
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.091 - 2.250
R-factor	0.1867
Rwork	0.186
R-free	0.21340
RMSD bond length	0.006
RMSD bond angle	0.755
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	26.091
High resolution limit [Å]	2.250
Number of reflections	42661
<I/σ(I)>	3.9
Completeness [%]	99.1
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG400, 50 mM magnesium acetate, 50 mM sodium acetate