5VJX
Crystal structure of the CLOCK Transcription Domain Exon19 in Complex with a Repressor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.536, 116.836, 132.926 |
| Unit cell angles | 90.00, 126.54, 90.00 |
Refinement procedure
| Resolution | 47.307 - 2.695 |
| R-factor | 0.2208 |
| Rwork | 0.218 |
| R-free | 0.27490 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.429 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.695 |
| Number of reflections | 59416 |
| <I/σ(I)> | 16.1 |
| Completeness [%] | 99.9 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 291 | 100mM Hepes pH 8.0, 200mM Proline, 15% PEG3350, 3% myo-Inositiol (w/v) |
