5VJF

Class II fructose-1,6-bisphosphate aldolase of Helicobacter pylori with DHAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-01
Detector	ADSC QUANTUM 4r
Wavelength(s)	1.0809
Spacegroup name	P 1 21 1
Unit cell lengths	39.518, 88.046, 90.811
Unit cell angles	90.00, 100.39, 90.00

Refinement procedure

Resolution	39.488 - 1.850
R-factor	0.1404
Rwork	0.137
R-free	0.17430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c4u
RMSD bond length	0.006
RMSD bond angle	0.924
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1134)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.488
High resolution limit [Å]	1.850
Number of reflections	50194
<I/σ(I)>	16.5
Completeness [%]	91.9
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	297	PEG 8000, PEG 1000, Calcium acetate, Tris-Acetic acid buffer