5VJC

TOG-tubulin binding specificity promotes microtubule dynamics and mitotic spindle formation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-10-17
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.03319
Spacegroup name	P 21 21 21
Unit cell lengths	53.256, 80.035, 146.771
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.327 - 2.000
R-factor	0.1887
Rwork	0.187
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2of3
RMSD bond length	0.008
RMSD bond angle	0.999
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.300	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.028	0.401
Number of reflections	43212	4251
<I/σ(I)>	17.7	2
Completeness [%]	95.6	86
Redundancy	2	2
CC(1/2)	0.992	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	26% PEG 3350 and 0.15 M Sodium Tartrate Dibasic Dihydrate