5VJ4
Crystal structure of a putative surface protein encoded by BTA121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-17 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 102.055, 102.055, 183.211 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.800 - 2.800 |
| R-factor | 0.19732 |
| Rwork | 0.196 |
| R-free | 0.22401 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.466 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.081 | 1.160 |
| Rpim | 0.037 | |
| Number of reflections | 26628 | 2665 |
| <I/σ(I)> | 20.3 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 5.7 |
| CC(1/2) | 0.738 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.8M lithium sulfate, 50mM sodium HEPES pH 7.5 |
