5VIK
Crystal structure of monomeric near-infrared fluorescent protein miRFP703
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.319, 52.821, 64.622 |
| Unit cell angles | 90.00, 92.53, 90.00 |
Refinement procedure
| Resolution | 29.930 - 1.350 |
| R-factor | 0.1232 |
| Rwork | 0.121 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xtq |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.324 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.930 | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 2.910 | 1.350 |
| Rmerge | 0.054 | 0.047 | 0.368 |
| Rmeas | 0.063 | 0.055 | 0.441 |
| Rpim | 0.033 | 0.028 | 0.241 |
| Total number of observations | 197471 | ||
| Number of reflections | 53575 | ||
| <I/σ(I)> | 13.6 | ||
| Completeness [%] | 99.9 | 99.5 | 99.9 |
| Redundancy | 3.7 | 3.7 | 3.3 |
| CC(1/2) | 0.994 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.08M Na Citrate pH 5.0, 24% v/v Jeffamine ED-2001 pH 7.0 0.4% n-octyl-beta-D-glucopyranoside |
