5VHU
E. coli CFT073 c3406
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-10-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.978 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 71.832, 71.489, 77.234 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.600 - 1.800 |
| R-factor | 0.17523 |
| Rwork | 0.174 |
| R-free | 0.20806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fxa |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.827 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 52.600 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 17785 |
| <I/σ(I)> | 10.7 |
| Completeness [%] | 99.8 |
| Redundancy | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 0.1 M sodium citrate tribasic pH 5.6, 0.5 M NaCl, 2% v/v ethylene imine polymer |
