5VHD
DHX36 with an N-terminal truncation bound to ADP-AlF4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.030, 112.500, 63.180 |
| Unit cell angles | 90.00, 110.33, 90.00 |
Refinement procedure
| Resolution | 46.734 - 2.550 |
| R-factor | 0.1744 |
| Rwork | 0.173 |
| R-free | 0.21310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeMet labeled protein |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.562 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.734 |
| High resolution limit [Å] | 2.550 |
| Rmerge | 0.149 |
| Rpim | 0.071 |
| Number of reflections | 26260 |
| <I/σ(I)> | 9.89 |
| Completeness [%] | 98.8 |
| Redundancy | 5.1 |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294.15 | 0.2 M potassium sodium tartrate and 20% w/v PEG 3350 |
