5VHA

DHX36 with an N-terminal truncation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	61.324, 109.191, 62.406
Unit cell angles	90.00, 112.71, 90.00

Refinement procedure

Resolution	39.614 - 2.227
R-factor	0.1789
Rwork	0.177
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SeMet labeled protein
RMSD bond length	0.004
RMSD bond angle	0.744
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.614
High resolution limit [Å]	2.227
Rmerge	0.087
Rpim	0.037
Number of reflections	37011
<I/σ(I)>	18.28
Completeness [%]	99.8
Redundancy	6.5
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294.15	0.2 M ammonium citrate tribasic pH 7.0 and 20% w/v PEG3350