5VGR
Human Atlastin-3, GDP-bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.6362 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.666, 57.057, 143.423 |
| Unit cell angles | 90.00, 116.22, 90.00 |
Refinement procedure
| Resolution | 29.305 - 2.096 |
| R-factor | 0.1986 |
| Rwork | 0.198 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q5e |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.051 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.305 | 2.171 |
| High resolution limit [Å] | 2.096 | 2.096 |
| Number of reflections | 55378 | 4912 |
| <I/σ(I)> | 12.43 | 1.14 |
| Completeness [%] | 98.4 | 88.17 |
| Redundancy | 4.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M NaCl, 0.1 M Tris pH 8.5, 25% PEG3350, 25% glycerol |
