5VFX
Structure of an accessory protein of the pCW3 relaxosome in complex with the origin of transfer (oriT) DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953700 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.503, 109.894, 122.780 |
| Unit cell angles | 90.00, 93.02, 90.00 |
Refinement procedure
| Resolution | 43.080 - 2.810 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vfy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.200 | 47.200 | 2.910 |
| High resolution limit [Å] | 2.810 | 10.500 | 2.810 |
| Rmerge | 0.165 | 0.034 | 2.100 |
| Rmeas | 0.192 | 0.040 | 2.458 |
| Rpim | 0.098 | 0.020 | 1.264 |
| Total number of observations | 166112 | 3144 | 15852 |
| Number of reflections | 44405 | ||
| <I/σ(I)> | 6.5 | 25 | 0.7 |
| Completeness [%] | 98.4 | 94.8 | 93.6 |
| Redundancy | 3.7 | 3.7 | 3.6 |
| CC(1/2) | 0.992 | 0.998 | 0.217 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M imidazole, 50% (v/v) 2-methyl-2,4-pentanediol |
